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a ej pcmv6 ac  (Addgene inc)


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    Structured Review

    Addgene inc a ej pcmv6 ac
    A Ej Pcmv6 Ac, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/a+ej+pcmv6+ac/pCMV6-AC+4-%CE%BCHOM+(Plasmid+%23113619)/pm41526379-357-18-21
    Average 94 stars, based on 3 article reviews
    a ej pcmv6 ac - by Bioz Stars, 2026-10
    94/100 stars

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    Stable Transfection:

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7-GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619), or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9-based DNA repair assays , cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID.
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619) or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9 based DNA repair assays 86, cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Transfection:

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7-GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619), or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9-based DNA repair assays , cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID.
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619) or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9 based DNA repair assays 86, cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Construct:

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7-GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619), or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9-based DNA repair assays , cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID.
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619) or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9 based DNA repair assays 86, cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Non-Homologous End Joining:

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7-GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619), or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9-based DNA repair assays , cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID.
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619) or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9 based DNA repair assays 86, cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Plasmid Preparation:

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7-GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619), or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9-based DNA repair assays , cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).

    Article Title: DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID.
    Article Snippet: .. CH12F3 cells were stably transfected with 2 μg of reporter constructs for NHEJ: pCMV6-AC EJ7GFP (Addgene plasmid #113617), A-EJ: pCMV6-AC 4-μHOM (Addgene plasmid #113619) or HR: pDR-GFP (Addgene plasmid #26475). .. For CRISPR/Cas9 based DNA repair assays 86, cells were transiently transfected with 1 μg each of 7a and 7b sgRNA plasmids for NHEJ (Addgene plasmid #113620 and #113624).



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